Coliforms are cultured in a specialized growth broth.  The culture broth contains proteins and a specialized buffer that guarantees the rapid growth of coliforms.  It also contains an inhibitor to prevent the growth of other bacteria, especially Gram-positive bacteria. To detect coliforms, a chromogenic substrate is added to the medium that turns the growth medium blue-green when metabolized by coliforms.   In addition the culture medium contains an E. coli specific fluorescent substrate that glows blue when exposed to UV light.  The coliform test can be either qualitative (positive/negative) or quantitative.

Best Uses

• When black water is released indoors, from flooding, or sewage backup
• Health care facilities where the environment directly impacts patient health
• Well water—all wells should be tested annually for the presence of coliforms
• Fecal contamination indoors due to pests such as mice, rats, birds, bats
• Fecal contamination indoors due to pet activity
• To evaluate the quality of janitorial services

Key Coliform Facts

• 48 hour turnaround time.
• Robotic Handling and Advanced Image Analysis
• Analysts Have Bachelor Degrees or Higher
• Overnight Shipping to the Lab
• Ph.D. Microbiologist on Staff
• Free Phone Consultation with Results
• Results either qualitative or quantitative based on need.
• High sensitive for detecting coliforms.

Interferences

• Desiccation—excessive drying during collection may lead to loss of viability
• Environmental extremes—avoid excessive exposure to heat and direct sunlight
• Biocides and disinfectants—avoid collecting samples from areas recently treated with antimicrobials

Sample Collection

• Swab, dust, or bulk materials

• Water collected in a special container provided by AssuredBio

Downstream Assays

• Genus Identification
• Species Identification

Pricing

$25.00 Qualitative results (positive/negative)
$45.00 Quantitative results (coliform counts/E.coli counts)
Call for volume pricing.