DNA Testing allows for accurate and rapid detection of a wide variety of organisms. Assured Bio Labs is at the forefront of DNA testing being one of the first lab in the United States to be accredited for molecular detection of molds and bacteria by AIHA-LAP.


Mold Specific Quantitative Polymerase Chain Reaction is the umbrella term used to describe mold DNA testing based off the EPA’s ERMI research program. These include the ERMI, SIM, SIAM, PFP, ARMI, etc. When using dust collector samples you can get a look at the mold history in the home. If you follow the EPA’s ERMI protocol you can receive an ERMI score which is useful to compare one home against the mold burden in the average home. A SIM (Survey of Indoor Molds), which uses all the same technology as the ERMI, can be used  for other sample types that do not conform to the ERMI protocol. This analysis can be used with either a dust collector or swab. If you are interested in the current levels of indoor airborne mold, our proprietary M-TRAP® cassette can be used with an IAQ sampling pump which you can perform a SIAM (the ERMI panel for the air) on to get results similar to a spore trap but with species identifications.


  • DNA testing is fast: most analyses can be ran with same day turn around for additional cost.
  • DNA testing is flexible; we can sample from just about every sample matrix and can run many analyses from a single sample. We are also able to design custom testing for clients per their request.
  • DNA testing is accurate; some tests are accurate down to one cell per sample type. It also eliminates human error due to no actual human interaction in the analysis. This makes it a great choice for issues that may end up in court.
  • All these things and the fact that DNA testing is easy make it a great choice for anyone to use no matter their skill level or experience as an inspector.


View some of our MSQPCR analyses below 

Not finding the test you are looking for? Click on our  DNA Panels section below then fill out our contact form or give us a call at (865) 813-1700.

Contact us for Inspector Pricing.

The ERMI DNA mold analysis detects 36 common indoor molds as identified by the US-EPA (ERMI score included).

Contact us for Inspector Pricing.

DNA mold analysis that detects 36 common indoor molds as identified by the US-EPA (ERMI score NOT included).

Tests for molds highly correlated with water damage, high moisture levels, and toxic molds (Stachybotrys and Aspergillus/Penicillium).

Contact us for Inspector Pricing.

DNA mold analysis that detects 13 species of mold and provides a moldiness index of the building tested.

Analysis derived from the ERMI panel. Click to learn more.


We offer a wide range of bacterial testing, from a simple total bacteria screen (Universal Bacteria) to more specific tests like MRSA (Methicillin Resistant Staphylococcus aureus) and Legionella (causes Legionnaires’ Disease).


We also offer other types of DNA testing. We can test for the presence of Bed Bugs (Cimex lectularius) using PCR, Histoplasma/Cryptococcus (avian pathogens), and also Acanthamoeba (an infective amoeba). We test for a large variety organisms just check out our pricing list below. If you want to inquire about something you don’t see on the list feel free to give us a call. We have experience developing custom assays and special projects.


Standard turnaround time for DNA analyses are 48-72 hours. Same day Turnaround is available for most options below. Inquire for details.

D1. Environmental Relative Moldiness Index (ERMI)

Dust analysis using the EPA developed 36 assay panel with the ERMI Score

D2. Survey of Indoor Molds (SIM)

Dust, Swab, or Bulk analysis using the EPA’s ERMI 36 assay panel with no score

D3. Species Identification of Airborne Molds (SIAM)

MTrap® (more on MTrap® on page 23) cassette analysis using the EPA’s ERMI 36 assay panel

D4. American Relative Moldiness Index (ARMI)

Analysis using the EPA developed 13 assay panel with an ARMI Score

D5. Big 2 Analysis

Economy analysis for common indoor moisture intrusion indicating molds – Total Penicillium/Aspergillus and Stachybotrys chartarum

D6. Pathogenic Fungus Panel

Analysis for the 12 common pathogenic mold species from the ERMI panel (Astrc, Aaltr, Aflav, Afumi, Anigr, Austs2, Avers2-2, Cglob, Muc1, Pvari2, SCbrv, Tviri)

D7. Aspergillosis Assessment

Analysis for 12 common Aspergillus species from the ERMI panel (Aflav, Afumi, Anigr, Aochr1, Apeni2, Arest, Ascler, Asydo3, Aungu, Austs2, Avers2-2)

D8. Penicillium Assessment

Analysis for 7 common Penicillium species from the ERMI panel (Pbrev, Pchry, Pcory, PenGrp2, Psoin2, Ppurp, Pvarb2)


Analysis for a panel of common health concern molds from the ERMI panel (Aaltr, Anigr, Aflav, Afumi, Avers2-2, Cglob, Stac)

D10. CAP (2, 4, 8, 14).

Analysis using a 2, 4, 8, or 14 assay panel derived from the ERMI panel (Apull, Cclad1, Pbrev, Pchry, Anigr, Eamst, Afumi, Stac, Aflav, Apeni2, Avers2-2, Cglob, Tviri, Wsebi)

D11. Aflatoxin Test

Analysis for the two aflatoxin producing species Aspergillus flavus and Aspergillus parasiticus (Aflavsp, Apara)

D12. Staphylococcus aureus/MRSA qPCR Analysis

Staphylococcus aureus and Methicillin-resistant S. aureus (Stapha, MRSA)

D13. Pathogenic Bacteria Panel

Custom panel of common pathogenic bacteria Eshericia coli, Staphylococcus aures, MRSA, Clostridium difficile, Mycobacterium tuberculosis, Legionella species, and Listeria species

D14. Avian Pathogen Panel

Presence/Absence Histoplasma capsulatum and Cryptococcus neoformans/C. gattii (Histo, Crypto)

D15. Pathogenic Candida Panel

Candida auris and Candida albicans (Cauri, Calbi)

D16. Fecal Contamination Panel

Total Bacteroides species and Human Bacteroides (Indicator of Human Feces)

Assay Code Organism Quantitative or Presence Absence (P/A)
Ufun Universal Fungi Quantitative
PenAsp Total Penicillium/Aspergillus Quantitative
Astrc Acremonium strictum Quantitative
Aaltr Alternaria alternata Quantitative
Aflavsp Aspergillus flavus Quantitative
Aflav Aspergillus flavus/oryzae Quantitative
Afumi Aspergillus fumigatus, Neosartorya fischeri Quantitative
Anigr Aspergillus niger/awamori/foetidus/phoenicis Quantitative
Aochr1 Aspergillus ochraceus/ostianus Quantitative
Apara Aspergillus parasiticus Quantitative
Apeni2 Aspergillus penicillioides Quantitative
Arest Aspergillus restrictus/caesillus/conicus Quantitative
Asclr Aspergillus sclerotiorum Quantitative
Asydo3 Aspergillus sydowii Quantitative
Aungu Aspergillus unguis Quantitative
Austs2 Aspergillus ustus Quantitative
Avers2-2 Aspergillus versicolor Quantitative
Apull Aureobasidium pullulans Quantitative
Calbi Candida albicans Quantitative
Cauri Candida auris Quantitative
Cglob Chaetomium globosum Quantitative
Cclad1 Cladosporium cladosporioides svar. 1 Quantitative
Cclad2 Cladosporium cladosporioides svar. 2 Quantitative
Cherb Cladosporium herbarum Quantitative
Cspha Cladosporium sphaerospermum Quantitative
Crypto Cryptococcus neoformans/gattii P/A
Enigr Epicoccum nigrum Quantitative
Eamst Eurotium (Aspergillus) amstelodami/chevalieri/herbariorum/rubrum/


Histo Histoplasma capsulatum P/A
Muc1 Mucor amphibiorum/circinelloides/hiemalis/indicus/mucedo/racemosus/

ramosissimus and Rhizopus azygosporus/homothalicus/microsporus/ oligosporus/oryzae

Pvari2 Paecilomyces variotii Quantitative
Pbrev Penicillium brevicompactum/stoloniferum Quantitative
Pchry Penicillium chrysogenum Quantitative
Pcory Penicillium corylophilum Quantitative
PenGrp2 Penicillium crustosum/camemberti/commune/echinulatum/solitum Quantitative
Pspin2 Penicillium glabrum/lividum/purpurescens/spinulosum/thomii Quantitative
Ppurp Penicillium purpurogenum Quantitative
Pvarb2 Penicillium variabile Quantitative
Phoma Phoma species P/A
PM Powdery Mildew (Erysiphales) Quantitative
Rstol Rhizopus stolonifer Quantitative
SCbrv Scopulariopsis brevicaulis/fusca Quantitative
SCchr Scopulariopsis chartarum Quantitative
Stac Stachybotrys chartarum Quantitative
Tviri Trichoderma viride/atroviride/koningii Quantitative
Tmel Tuber melanosporum (Black Truffle) P/A
Wsebi Wallemia sebi Quantitative

Assay Code Organism Quantitative or Presence Absence (P/A)
Ubact Universal Bacteria Quantitative
TBact Total Bacteroides species P/A
HBact Human Bacteroides (Indicator of Human Fecal Contamination) P/A
Cdiff Clostridium difficile (C diff) Quantitative
EColi Eschericia coli (E coli) P/A
Lbz Legionella bozemanae Quantitative
Ldm Legionella dumoffii Quantitative
Lfe Legionella feeleii Quantitative
Llb Legionella longbeachae Quantitative
Lmd Legionella micdadei Quantitative
Lpn Legionella pneumophila (all serogroups) Quantitative
Lpns1 Legionella pneumophila serogroup 1 Quantitative
Lsp Legionella species Quantitative
Lis-mon Listeria monocytogenes P/A
Lis-all Listeria species P/A
MRSA Methicillin-resistant Staphylococcus aureus (MRSA) Quantitative
Mtb Mycobacterium tuberculosis Quantitative
Sal Salmonella species P/A
Stapha Staphylococcus aureus Quantitative
Assay Code Organism Quantitative or Presence Absence (P/A)
Acan Acanthamoeba species P/A
BB Bed Bug (Cimex lectularius) P/A
InflA Influenza A Quantitative
InflB Influenza B Quantitative


ERMI Analysis

The Environmental Relative Moldiness Index (ERMI) is a Quantitative, Real-Time Polymerase Chain Reaction (QPCR) panel of testing for indoor molds that was developed by the United States Environmental Protection Agency (US-EPA). This panel includes two groups of molds. Group one contains mold species and groups of species that are known to thrive in water damaged homes. The second group detects 10 species or groups of species of molds that are found in all homes, with or without water damage. Each species and group of species is enumerated from DNA extracted from dust samples taken from both the living and sleeping quarters of the home. Concentrations of each of the 36 assays are used to derive an “ERMI score” that rates the moldiness of each sample against those tested by the US-EPA. These values range from approximately –10 (low moldiness) to 20 (high moldiness) with an average home coming in with a score of “0”.

U.S. EPA License #801-14

Best Uses

ERMI is ideal for buildings with health concerns, legal issues, and detection of hidden mold problems. It is used in homes, offices, schools, and other buildings, public and private.

Key ERMI Facts

  • 99.99% Accurate
  • Species Identification of 36 Types of Mold
  • Many Assays Detect the Presence of a Single Spore
  • Requires No Outdoor Control Sample
  • Results Are Comparable to National Averages
  • Requires Only 1 Sample Per Living Space
  • Rapidly Differentiates Between Aspergillus and Penicillium-like species of mold
  • Provides Historical Perspective Other Tests Cannot
  • Results are Extremely Easy to Understand

Sample Collection

Requires a standard vacuum cleaner and dust collection device. A 6’x3′ area is vacuumed for five minutes in two frequently occupied rooms such as a living room and master bedroom. Carpeting is not necessary and wood, tile or laminate floors may also be sampled.
Included Species

  • Aspergillus fumigatus, Neosartorya fischeri
    Aspergillus ochraceus / ostianus
    Aspergillus restrictus / caesillus / conicus
    Aspergillus sclerotiorum
    Aspergillus unguis
    Aspergillus versicolor
    Aspergillus penicillioides
    Cladosporium sphaerospermum
    Eurotium (Aspergillus) amstelodami / chevalieri / herbariorum / rubrum / repens
    Penicillium purpurogenum
    Stachybotrys chartarum
    Aspergillus flavus / oryzae
    Aspergillus niger / awamori / foetidus / phoenicis
    Aspergillus sydowii
    Aureobasidium pullulans
    Chaetomium globosum
    Paecilomyces variotii
    Penicillium brevicompactum / stoloniferum
    Penicillium corylophilum
    Penicillium crustosum / camembertii / commune / echinulatum / solitum
    Penicillium glabrum / lividum / purpurescens / spinulosum / thomii
    Penicillium variable
    Scopulariopsis brevicaulis / fusca
    Scopulariopsis chartarum
    Trichoderma viride / atroviride / koningii
    Wallemia sebi
    Acremonium strictum
    Alternaria alternata
    Cladosporium cladosporioides svar. 1
    Cladosporium cladosporioides svar. 2
    Cladosporium herbarum
    Aspergillus ustus
    Epicoccum nigrum
    Mucor amphibiorum / circinelloides / hiemalis / indicus / mucedo / racemosus / ramosissimus and Rhizopus azygosporus / homothalicus / microsporus / oligosporus / oryzae
    Penicillium chrysogenum
    Rhizopus stolonifer

Other Mold Specific QPCR Analysis

For custom MSQPCR panels, contact the lab.

Survey of Indoor Molds (SIM)

The Survey of Indoor Molds (SIM) is a collection of assays employing Mold Specific Quantitative Polymerase Chain Reaction (MSQPCR) technology that was developed by the United States Environmental Protection Agency. The SIM is a panel designed by AssuredBio to appeal to industrial hygienists and other highly trained and experienced individuals. As opposed to emphasizing a generalized score, the SIM emphasizes individual species quantification because they will be used by the hygienist in forming recommendations and strategies for remediation. Most species of fungi occurring indoors are found worldwide in soils, rendering them useful indicators of indoor conditions in any location. Additionally, mycoses (fungal infections) have been documented for some of the species in both healthy and immunocompromised people.

Aspergillus Assessment Panel (AAP)

The genus Aspergillus contains over 185 species to which humans are constantly exposed in dust, decomposing organic matter, water and indoor air. Aspergillus species linked to mycoses include A. fumigatus, A. flavus, A. niger, A. nidulans, A. terreus, A. oryzae, A. ustus and A. versicolor. Aspergilli can be primary (capable of infecting healthy individuals) or opportunistic (causing infection in immunocompromised patients) pathogens. Infections can be nosocomial (hospital derived) or can result from inhalation of spores in ones everyday environment. Most cases of primary deep mycoses are asymptomatic or clinically mild infections occurring in normal patients living or traveling in endemic areas. However, patients exposed to a high numbers of organisms or those with weakened immune response may suffer life-threatening progression or reactivation of latent infections.

AssuredBio derived the Aspergillosis Assessment Panel (AAP) to provide investigators with the most thorough DNA-based assessment of the species that belong to the genus Aspergillus. Twelve species of Aspergillus are included in the AAP. A detailed description is provided for each species.

Pathogenic Fungus Panel (PFP)

Mycoses are infections caused by fungi and have been found in/on the brain, heart, lungs, liver, spleen, kidneys and skin. Primary fungal pathogens can establish infections in normal hosts, whereas opportunistic fungal pathogens can cause disease in individuals with compromised immune mechanisms. Infectious fungi may be either inside (endogenous) or outside (exogenous) of the body. Routes of entry for exogenous fungi include airborne spores and mold on surfaces. Endogenous infections are caused by fungi that are part of the body’s normal flora or by reactivation of a dormant infectious fungus residing in an old infection site. Invasive mycoses are difficult to diagnose and treat, resulting in a high mortality rate among affected patients.

The Pathogenic Fungus Panel (PFP) is a panel that was developed by AssuredBio under the guidance of Dr. Wiley Schell, a well known Medical Mycologist at Duke University Medical Center in Raleigh, NC. The panel contains a group of 12 molds that are known to cause disease in humans. Furthermore, the molds in the panel are separated into 5 groups based on medical significance. A detailed medical description of each mold target is provided.

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