DNA Testing allows for accurate and rapid detection of a wide variety of organisms. Assured Bio Labs is at the forefront of DNA testing being one of the first lab in the United States to be accredited for molecular detection of molds and bacteria by AIHA-LAP.
What Makes DNA Testing Better
- DNA testing is fast: most analyses can be ran with same day turn around for additional cost.
- DNA testing is flexible; we can sample from just about every sample matrix and can run many analyses from a single sample. We are also able to design custom testing for clients per their request.
- DNA testing is accurate; some tests are accurate down to one cell per sample type. It also eliminates human error due to no actual human interaction in the analysis. This makes it a great choice for issues that may end up in court.
- All these things and the fact that DNA testing is easy make it a great choice for anyone to use no matter their skill level or experience as an inspector.
Mold Specific Quantitative Polymerase Chain Reaction is the umbrella term used to describe mold DNA testing based off the EPA’s ERMI research program. These include the ERMI, SIM, SIAM, PFP, ARMI, etc. When using dust collector samples you can get a look at the mold history in the home. If you follow the EPA’s ERMI protocol you can receive an ERMI score which is useful to compare one home against the mold burden in the average home. A SIM (Survey of Indoor Molds), which uses all the same technology as the ERMI, can be used for another sample types that do not conform to the ERMI protocol. If you are interested in the current levels of indoor airborne mold, our proprietary MTrap® cassette can be used with an IAQ sampling pump which you can perform a SIAM (the ERMI panel for the air) on to get results similar to a spore trap but with species identifications.
We offer a wide range of bacterial testing, from a simple total bacteria screen (Universal Bacteria) to more specific tests like MRSA (Methicillin Resistant Staphylococcus aureus) and Legionella (causes Legionnaires’ Disease).
Bed Bugs, Avian Pathogen, etc
We also offer other types of DNA testing. We can test for the presence of Bed Bugs (Cimex lectularius) using PCR, Histoplasma/Cryptococcus (avian pathogens), and also Acanthamoeba (an infective amoeba). We test for a large variety organisms just check out our pricing list below. If you want to inquire about something you don’t see on the list feel free to give us a call. We have experience developing custom assays and special projects.
Standard turnaround time for DNA analyses are 48-72 hours. Same day Turnaround is available for most options below. Inquire for details.
D1. Environmental Relative Moldiness Index (ERMI)
Dust analysis using the EPA developed 36 assay panel with the ERMI Score
D2. Survey of Indoor Molds (SIM)
Dust, Swab, or Bulk analysis using the EPA’s ERMI 36 assay panel with no score
D3. Species Identification of Airborne Molds (SIAM)
MTrap® (more on MTrap® on page 23) cassette analysis using the EPA’s ERMI 36 assay panel
D4. American Relative Moldiness Index (ARMI)
Analysis using the EPA developed 13 assay panel with an ARMI Score
D5. Big 2 Analysis
Economy analysis for common indoor moisture intrusion indicating molds – Total Penicillium/Aspergillus and Stachybotrys chartarum
D6. Pathogenic Fungus Panel
Analysis for the 12 common pathogenic mold species from the ERMI panel (Astrc, Aaltr, Aflav, Afumi, Anigr, Austs2, Avers2-2, Cglob, Muc1, Pvari2, SCbrv, Tviri)
D7. Aspergillosis Assessment
Analysis for 12 common Aspergillus species from the ERMI panel (Aflav, Afumi, Anigr, Aochr1, Apeni2, Arest, Ascler, Asydo3, Aungu, Austs2, Avers2-2)
D8. Penicillium Assessment
Analysis for 7 common Penicillium species from the ERMI panel (Pbrev, Pchry, Pcory, PenGrp2, Psoin2, Ppurp, Pvarb2)
Analysis for a panel of common health concern molds from the ERMI panel (Aaltr, Anigr, Aflav, Afumi, Avers2-2, Cglob, Stac)
D10. CAP (2, 4, 8, 14).
Analysis using a 2, 4, 8, or 14 assay panel derived from the ERMI panel (Apull, Cclad1, Pbrev, Pchry, Anigr, Eamst, Afumi, Stac, Aflav, Apeni2, Avers2-2, Cglob, Tviri, Wsebi)
D11. Aflatoxin Test
Analysis for the two aflatoxin producing species Aspergillus flavus and Aspergillus parasiticus (Aflavsp, Apara)
D12. Staphylococcus aureus/MRSA qPCR Analysis
Staphylococcus aureus and Methicillin-resistant S. aureus (Stapha, MRSA)
D13. Pathogenic Bacteria Panel
Custom panel of common pathogenic bacteria Eshericia coli, Staphylococcus aures, MRSA, Clostridium difficile, Mycobacterium tuberculosis, Legionella species, and Listeria species
D14. Avian Pathogen Panel
Presence/Absence Histoplasma capsulatum and Cryptococcus neoformans/C. gattii (Histo, Crypto)
D15. Pathogenic Candida Panel
Candida auris and Candida albicans (Cauri, Calbi)
D16. Fecal Contamination Panel
Total Bacteroides species and Human Bacteroides (Indicator of Human Feces)
Individual Fungi Analyses
|Assay Code||Organism||Quantitative or Presence Absence (P/A)|
|Afumi||Aspergillus fumigatus, Neosartorya fischeri||Quantitative|
|Cclad1||Cladosporium cladosporioides svar. 1||Quantitative|
|Cclad2||Cladosporium cladosporioides svar. 2||Quantitative|
|Eamst||Eurotium (Aspergillus) amstelodami/chevalieri/herbariorum/rubrum/
ramosissimus and Rhizopus azygosporus/homothalicus/microsporus/ oligosporus/oryzae
|PM||Powdery Mildew (Erysiphales)||Quantitative|
|Tmel||Tuber melanosporum (Black Truffle)||P/A|
Individual Bacteria Analyses
|Assay Code||Organism||Quantitative or Presence Absence (P/A)|
|TBact||Total Bacteroides species||P/A|
|HBact||Human Bacteroides (Indicator of Human Fecal Contamination)||P/A|
|Cdiff||Clostridium difficile (C diff)||Quantitative|
|EColi||Eschericia coli (E coli)||P/A|
|Lpn||Legionella pneumophila (all serogroups)||Quantitative|
|Lpns1||Legionella pneumophila serogroup 1||Quantitative|
|MRSA||Methicillin-resistant Staphylococcus aureus (MRSA)||Quantitative|
Other Individual Analyses
|Assay Code||Organism||Quantitative or Presence Absence (P/A)|
|BB||Bed Bug (Cimex lectularius)||P/A|
The Environmental Relative Moldiness Index (ERMI) is a Quantitative, Real-Time Polymerase Chain Reaction (QPCR) panel of testing for indoor molds that was developed by the United States Environmental Protection Agency (US-EPA). This panel includes two groups of molds. Group one contains mold species and groups of species that are known to thrive in water damaged homes. The second group detects 10 species or groups of species of molds that are found in all homes, with or without water damage. Each species and group of species is enumerated from DNA extracted from dust samples taken from both the living and sleeping quarters of the home. Concentrations of each of the 36 assays are used to derive an “ERMI score” that rates the moldiness of each sample against those tested by the US-EPA. These values range from approximately –10 (low moldiness) to 20 (high moldiness) with an average home coming in with a score of “0”.
U.S. EPA License #801-14
ERMI is ideal for buildings with health concerns, legal issues, and detection of hidden mold problems. It is used in homes, offices, schools, and other buildings, public and private.
Key ERMI Facts
- 99.99% Accurate
- Species Identification of 36 Types of Mold
- Many Assays Detect the Presence of a Single Spore
- Requires No Outdoor Control Sample
- Results Are Comparable to National Averages
- Requires Only 1 Sample Per Living Space
- Rapidly Differentiates Between Aspergillus and Penicillium-like species of mold
- Provides Historical Perspective Other Tests Cannot
- Results are Extremely Easy to Understand
Requires a standard vacuum cleaner and dust collection device. A 6’x3′ area is vacuumed for five minutes in two frequently occupied rooms such as a living room and master bedroom. Carpeting is not necessary and wood, tile or laminate floors may also be sampled.
- Aspergillus fumigatus, Neosartorya fischeri
- Aspergillus ochraceus / ostianus
- Aspergillus restrictus / caesillus / conicus
- Aspergillus sclerotiorum
- Aspergillus unguis
- Aspergillus versicolor
- Aspergillus penicillioides
- Cladosporium sphaerospermum
- Eurotium (Aspergillus) amstelodami / chevalieri / herbariorum / rubrum / repens
- Penicillium purpurogenum
- Stachybotrys chartarum
- Aspergillus flavus / oryzae
- Aspergillus niger / awamori / foetidus / phoenicis
- Aspergillus sydowii
- Aureobasidium pullulans
- Chaetomium globosum
- Paecilomyces variotii
- Penicillium brevicompactum / stoloniferum
- Penicillium corylophilum
- Penicillium crustosum / camembertii / commune / echinulatum / solitum
- Penicillium glabrum / lividum / purpurescens / spinulosum / thomii
- Penicillium variable
- Scopulariopsis brevicaulis / fusca
- Scopulariopsis chartarum
- Trichoderma viride / atroviride / koningii
- Wallemia sebi
- Acremonium strictum
- Alternaria alternata
- Cladosporium cladosporioides svar. 1
- Cladosporium cladosporioides svar. 2
- Cladosporium herbarum
- Aspergillus ustus
- Epicoccum nigrum
- Mucor amphibiorum / circinelloides / hiemalis / indicus / mucedo / racemosus / ramosissimus and Rhizopus azygosporus / homothalicus / microsporus / oligosporus / oryzae
- Penicillium chrysogenum
- Rhizopus stolonifer
Other Mold Specific QPCR Analysis
For custom MSQPCR panels, contact the lab.
Survey of Indoor Molds (SIM)
The Survey of Indoor Molds (SIM) is a collection of assays employing Mold Specific Quantitative Polymerase Chain Reaction (MSQPCR) technology that was developed by the United States Environmental Protection Agency. The SIM is a panel designed by AssuredBio to appeal to industrial hygienists and other highly trained and experienced individuals. As opposed to emphasizing a generalized score, the SIM emphasizes individual species quantification because they will be used by the hygienist in forming recommendations and strategies for remediation. Most species of fungi occurring indoors are found worldwide in soils, rendering them useful indicators of indoor conditions in any location. Additionally, mycoses (fungal infections) have been documented for some of the species in both healthy and immunocompromised people.
Aspergillus Assessment Panel (AAP)
The genus Aspergillus contains over 185 species to which humans are constantly exposed in dust, decomposing organic matter, water and indoor air. Aspergillus species linked to mycoses include A. fumigatus, A. flavus, A. niger, A. nidulans, A. terreus, A. oryzae, A. ustus and A. versicolor. Aspergilli can be primary (capable of infecting healthy individuals) or opportunistic (causing infection in immunocompromised patients) pathogens. Infections can be nosocomial (hospital derived) or can result from inhalation of spores in ones everyday environment. Most cases of primary deep mycoses are asymptomatic or clinically mild infections occurring in normal patients living or traveling in endemic areas. However, patients exposed to a high numbers of organisms or those with weakened immune response may suffer life-threatening progression or reactivation of latent infections.
AssuredBio derived the Aspergillosis Assessment Panel (AAP) to provide investigators with the most thorough DNA-based assessment of the species that belong to the genus Aspergillus. Twelve species of Aspergillus are included in the AAP. A detailed description is provided for each species.
Pathogenic Fungus Panel (PFP)
Mycoses are infections caused by fungi and have been found in/on the brain, heart, lungs, liver, spleen, kidneys and skin. Primary fungal pathogens can establish infections in normal hosts, whereas opportunistic fungal pathogens can cause disease in individuals with compromised immune mechanisms. Infectious fungi may be either inside (endogenous) or outside (exogenous) of the body. Routes of entry for exogenous fungi include airborne spores and mold on surfaces. Endogenous infections are caused by fungi that are part of the body’s normal flora or by reactivation of a dormant infectious fungus residing in an old infection site. Invasive mycoses are difficult to diagnose and treat, resulting in a high mortality rate among affected patients.
The Pathogenic Fungus Panel (PFP) is a panel that was developed by AssuredBio under the guidance of Dr. Wiley Schell, a well known Medical Mycologist at Duke University Medical Center in Raleigh, NC. The panel contains a group of 12 molds that are known to cause disease in humans. Furthermore, the molds in the panel are separated into 5 groups based on medical significance. A detailed medical description of each mold target is provided.