What is qPCR?
Quantitative Polymerase Chain Reaction (qPCR) is a molecular technique pivotal for the detection and quantification of bacterial DNA. Operating on the principles of PCR, qPCR involves the real-time amplification of specific DNA regions, with fluorescence monitoring during the process. This allows for the simultaneous identification and quantification of bacterial species based on targeted DNA sequences. The method’s sensitivity permits the detection of low concentrations of bacterial DNA, facilitating early identification of contamination.
In application, qPCR’s real-time monitoring and ability to amplify multiple DNA targets concurrently make it invaluable for comprehensive microbial analysis. Its role spans diverse sectors, offering a powerful tool for researchers and practitioners seeking quantitative data on bacterial load, species identification, and early detection of microbial contamination.